Active substances & antimicrobial agents
Standard / Test method
ASTM E2149
Testing of the antimicrobial activity of antimicrobial agents and treated materials under dynamic contact conditions in accordance with ASTM E2149.
Overview
ASTM E2149 describes a standardised test method for the quantitative determination of antimicrobial activity under dynamic contact conditions.
The shake-flask suspension method is particularly suitable for assessing substrate-bound antimicrobial agents and antimicrobially treated materials.
Typical applications include screening tests and quality control. A typical test organism is Escherichia coli. Results are evaluated quantitatively.
Testing can be adapted for the specific project, depending on the testing objective, sample type, sample size, contact time or selected test strains.
Typical applications
Antimicrobial agent screening
For assessing and comparing antimicrobial agents under defined test conditions.
Material development
For assessing functionalised materials and new antimicrobial product systems.
Quality control
For comparative testing and verification of antimicrobial properties.
Frequently asked questions
Which products is ASTM E2149 suitable for?
ASTM E2149 is particularly suitable for antimicrobial agents, functionalised materials and products whose antimicrobial activity is to be assessed under dynamic contact conditions.
What does "dynamic contact conditions" mean?
In ASTM E2149, testing is carried out in an agitated suspension. Agitation promotes contact between microorganisms and the test material throughout the test period.
Which test organisms are used in ASTM E2149?
Escherichia coli is typically used. Depending on the testing objective, additional test organisms can be considered for the specific project.
What is the difference between ASTM E2149 and ASTM E2180?
ASTM E2149 assesses antimicrobial activity under dynamic contact conditions in a suspension. ASTM E2180 is commonly used for polymeric or hydrophobic material surfaces.