Antimicrobial agents
Standard / Test method
ASTM E2315
Testing of the antimicrobial activity of antimicrobial agents and liquid formulations using a time-kill procedure following ASTM E2315.
Overview
ASTM E2315 provides guidance on time-kill testing for the quantitative assessment of the antimicrobial activity of antimicrobial agents and products.
Typical applications include disinfectants, oral care products, wound care products and other liquid formulations.
Efficacy is assessed by determining the reduction in viable microorganisms after defined contact times. Results are evaluated quantitatively in terms of the log reduction in viable microbial counts.
Test conditions can be adapted for the specific project, depending on the testing objective, sample type, product concentration, contact times or selected test strains.
Typical applications
Disinfectants
Assessment of the antimicrobial efficacy of liquid disinfectants at defined contact times.
Product development
Comparison of different antimicrobial agent concentrations and formulations during the development of new products.
Efficacy assessment
Quantitative determination of microbial reduction for antimicrobial products and active substance systems.
Frequently asked questions
What is a time-kill test?
A time-kill test assesses the antimicrobial efficacy of a product over time. The number of viable microorganisms is determined after defined contact times and compared with the initial count.
Which products is ASTM E2315 suitable for?
The procedure is particularly suitable for disinfectants, antibacterial formulations, oral care products, wound care products and other liquid antimicrobial products.
How is efficacy assessed?
Results are evaluated quantitatively based on microbial reduction or log reduction after defined contact times.
What is the difference between ASTM E2315 and MIC testing?
ASTM E2315 assesses the reduction in viable microorganisms caused by a product over time. MIC testing based on DIN 58940-8 determines the lowest concentration of an antimicrobial agent that prevents visible growth.